Mitsunobu reaction

The Mitsunobu reaction is an organic reaction that converts an alcohol into a variety of functional groups, such as an ester, using triphenylphosphine and an azodicarboxylate such as diethyl azodicarboxylate (DEAD) or diisopropyl azodicarboxylate (DIAD).[1] The alcohol undergoes an inversion of stereochemistry. It was discovered by Oyo Mitsunobu (1934–2003).

Mitsunobu reaction
Named after Oyo Mitsunobu
Reaction type Coupling reaction
Organic Chemistry Portal mitsunobu-reaction
RSC ontology ID RXNO:0000034

Several reviews have been published.[2][3][4][5][6]

Reaction mechanism

The reaction mechanism of the Mitsunobu reaction is fairly complex. The identity of intermediates and the roles they play has been the subject of debate.

Initially, the triphenyl phosphine (2) makes a nucleophilic attack upon diethyl azodicarboxylate (1) producing a betaine intermediate 3, which deprotonates the carboxylic acid (4) to form the ion pair 5. DEAD itself deprotonates the alcohol (6) forming an alkoxide that can form the key oxyphosphonium ion 8. The ratio and interconversion of intermediates 811 depend on the carboxylic acid pKa and the solvent polarity.[7][8][9] Although several phosphorus intermediates are present, the attack of the carboxylate anion upon intermediate 8 is the only productive pathway forming the desired product 12 and triphenylphosphine oxide (13).

Hughes et al. have found that the formation of the ion pair 5 is very fast. The formation of the oxyphosphonium intermediate 8 is slow and facilitated by the alkoxide. Therefore, the overall rate of reaction is controlled by carboxylate basicity and solvation.[10]

Order of addition of reagents

The order of addition of the reagents of the Mitsunobu reaction can be important. Typically, one dissolves the alcohol, the carboxylic acid, and triphenylphosphine in tetrahydrofuran or other suitable solvent (e.g. diethyl ether), cool to 0 °C using an ice-bath, slowly add the DEAD dissolved in THF, then stir at room temperature for several hours. If this is unsuccessful, then preforming the betaine may give better results. To preform the betaine, add DEAD to triphenylphosphine in tetrahydrofuran at 0 °C, followed by the addition of the alcohol and finally the acid.[11]


Other nucleophilic functional groups

Many other functional groups can serve as nucleophiles besides carboxylic acids. For the reaction to be successful, the nucleophile must have a pKa less than 15.

Nucleophile Product
hydrazoic acid alkyl azide
imide substituted imide[12]
phenol alkyl aryl ether (discovered independently [13][14])
sulfonamide substituted sulfonamide[15]
arylsulfonylhydrazine alkyldiazene (subject to pericyclic or free radical dediazotization to give allene (Myers allene synthesis) or alkane (Myers deoxygenation), respectively)[16]


Several modifications to the original reagent combination have been developed in order to simplify the separation of the product and avoid production of so much chemical waste. One variation of the Mitsunobu Reaction uses resin-bound triphenylphoshine and uses di-tert-butylazodicarboxylate instead of DEAD. The oxidized triphenylphosphine resin can be removed by filtration, and the di-tert-butylazodicarboxylate byproduct is removed by treatment with trifluoroacetic acid.[17] Bruce H. Lipshutz has developed an alternative to DEAD, Di-(4-chlorobenzyl)azodicarboxylate (DCAD) where the hydrazine by-product can be easily removed by filtration and recycled back to DCAD.[18]

A modification has also been reported in which DEAD can be used in catalytic versus stoichiometric quantities, however this procedure requires the use of stoichiometric (diacetoxyiodo)benzene to oxidise the hydrazine by-product back to DEAD.[19]

Denton and co-workers have reported a redox-neutral variant of the Mitsunobu reaction which employs a phosphorus(III) catalyst to activate the substrate, ensuring inversion in the nucleophilic attack, and uses a Dean-Stark trap to remove the water by-product.[20]

Phosphorane reagents

Tsunoda et al. have shown that one can combine the triphenylphosphine and the diethyl azodicarboxylate into one reagent: a phosphorane ylide. Both (cyanomethylene)trimethylphosphorane (CMMP, R = Me) and (cyanomethylene)tributylphosphorane (CMBP, R = Bu) have proven particularly effective.[21]

The ylide acts as both the reducing agent and the base. The byproducts are acetonitrile (6) and the trialkylphosphine oxide (8).


The Mitsunobu reaction has been applied in the synthesis of aryl ethers:[22]

With these particular reactants the conversion with DEAD fails because the phenol is only weakly acidic. Instead the related 1,1'-(azodicarbonyl)dipiperidine (ADDP) is used of which the betaine intermediate is a stronger base. The phosphine is a polymer-supported triphenylphosphine (PS-PPh3).

The reaction has been used to synthesize quinine, colchicine, sarain, morphine, stigmatellin, eudistomin, oseltamivir, strychnine, and nupharamine.[23]


  1. Mitsunobu, O.; Yamada, Y. (1967). "Preparation of Esters of Carboxylic and Phosphoric Acid via Quaternary Phosphonium Salts". Bulletin of the Chemical Society of Japan. 40 (10): 2380–2382. doi:10.1246/bcsj.40.2380.
  2. Mitsunobu, O. (1981). "The Use of Diethyl Azodicarboxylate and Triphenylphosphine in Synthesis and Transformation of Natural Products". Synthesis. 1981 (1): 1–28. doi:10.1055/s-1981-29317.
  3. Castro, B. R. (1983). Replacement of Alcoholic Hydroxy Groups by Halogens and Other Nucleophiles via Oxyphosphonium Intermediates. Organic Reactions. 29. pp. 1–162. doi:10.1002/0471264180.or029.01. ISBN 9780471264187.
  4. Hughes, D. L. (1992). The Mitsunobu Reaction. Organic Reactions. 42. pp. 335–656. doi:10.1002/0471264180.or042.02. ISBN 9780471264187.
  5. Hughes, D. L. (1996). "Progress in the Mitsunobu Reaction. A Review". Organic Preparations and Procedures International. 28 (2): 127–164. doi:10.1080/00304949609356516.
  6. Swamy, K. C. K.; Kumar, N. N. B.; Balaraman, E. & Kumar, K. V. P. P. (2009). "Mitsunobu and Related Reactions: Advances and Applications". Chemical Reviews. 109 (6): 2551–2651. doi:10.1021/cr800278z. PMID 19382806.
  7. Grochowski, E.; Hilton, B. D.; Kupper, R. J.; Michejda, C. J. (1982). "Mechanism of the triphenylphosphine and diethyl azodicarboxylate induced dehydration reactions (Mitsunobu reaction). The central role of pentavalent phosphorus intermediates". Journal of the American Chemical Society. 104 (24): 6876–6877. doi:10.1021/ja00388a110.
  8. Camp, D.; Jenkins, I. D. (1989). "The mechanism of the Mitsunobu esterification reaction. Part I. The involvement of phosphoranes and oxyphosphonium salts". The Journal of Organic Chemistry. 54 (13): 3045–3049. doi:10.1021/jo00274a016.
  9. Camp, D.; Jenkins, I. D. (1989). "The mechanism of the Mitsunobu esterification reaction. Part II. The involvement of (acyloxy)alkoxyphosphoranes". The Journal of Organic Chemistry. 54 (13): 3049–3054. doi:10.1021/jo00274a017.
  10. Hughes, D. L.; Reamer, R. A.; Bergan, J. J.; Grabowski, E. J. J. (1988). "A mechanistic study of the Mitsunobu esterification reaction". Journal of the American Chemical Society. 110 (19): 6487–6491. doi:10.1021/ja00227a032.
  11. Volante, R. (1981). "A new, highly efficient method for the conversion of alcohols to thiolesters and thiols". Tetrahedron Letters. 22 (33): 3119–3122. doi:10.1016/S0040-4039(01)81842-6.
  12. Hegedus, L. S.; Holden, M. S.; McKearin, J. M. (1984). "cis-N-TOSYL-3-METHYL-2-AZABICYCLO[3.3.0]OCT-3-ENE". Organic Syntheses. 62: 48.CS1 maint: multiple names: authors list (link); Collective Volume, 7, p. 501
  13. Manhas, Maghar S.; Hoffman, W. H.; Lal, Bansi; Bose, Ajay K. (1975). "Steroids. Part X. A convenient synthesis of alkyl aryl ethers". J. Chem. Soc., Perkin Trans. 1 (5): 461–463. doi:10.1039/P19750000461.
  14. Bittner, Shmuel; Assaf, Yonit (1975). "Use of activated alcohols in the formation of aryl ethers". Chemistry & Industry (6): 281.
  15. Kurosawa, W.; Kan, T.; Fukuyama, T. (2002). "PREPARATION OF SECONDARY AMINES FROM PRIMARY AMINES VIA 2-NITROBENZENESULFONAMIDES: N-(4-METHOXYBENZYL)-3-PHENYLPROPYLAMINE". Organic Syntheses. 79: 186.CS1 maint: multiple names: authors list (link); Collective Volume, 10, p. 482.
  16. Myers, Andrew G.; Zheng, Bin (1996). "New and Stereospecific Synthesis of Allenes in a Single Step from Propargylic Alcohols". Journal of the American Chemical Society. 118 (18): 4492–4493. doi:10.1021/ja960443w. ISSN 0002-7863.
  17. Pelletier, J. C.; Kincaid, S. (2000). "Mitsunobu reaction modifications allowing product isolation without chromatography: application to a small parallel library". Tetrahedron Letters. 41 (6): 797–800. doi:10.1016/S0040-4039(99)02214-5.
  18. Lipshutz, B. H.; Chung, D. W.; Rich. B.; Corral, R. (2006). "Simplification of the Mitsunobu Reaction. Di-p-chlorobenzyl Azodicarboxylate: A New Azodicarboxylate". Organic Letters. 8 (22): 5069–5072. doi:10.1021/ol0618757. PMID 17048845.
  19. But, T. Y.; Toy, P. H. (2006). "Organocatalytic Mitsunobu Reactions". Journal of the American Chemical Society. 128 (30): 9636–9637. doi:10.1021/ja063141v. PMID 16866510.
  20. Beddoe, Rhydian H.; Andrews, Keith G.; Magné, Valentin; Cuthbertson, James D.; Saska, Jan; Shannon-Little, Andrew L.; Shanahan, Stephen E.; Sneddon, Helen F.; Denton, Ross M. (30 August 2019). "Redox-neutral organocatalytic Mitsunobu reactions". Science. 365 (6456): 910–914. doi:10.1126/science.aax3353. ISSN 0036-8075.
  21. Tsunoda, T.; Nagino, C.; Oguri, M.; Itô, S. (1996). "Mitsunobu-type alkylation with active methine compounds". Tetrahedron Letters. 37 (14): 2459–2462. doi:10.1016/0040-4039(96)00318-8.
  22. Humphries, P. S.; Do, Q. -Q. T.; Wilhite, D. M. (2006). "ADDP and PS-PPh3: an efficient Mitsunobu protocol for the preparation of pyridine ether PPAR agonists". Beilstein Journal of Organic Chemistry. 2 (21): 21. doi:10.1186/1860-5397-2-21. PMC 1705810. PMID 17076898.
  23. Mitsunobu Reaction at SynArchive Accessed April 26, 2014

See also

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