List of breast cancer cell lines

Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. This is a list of major breast cancer cell lines that are primarily used in breast cancer research.[Notes 1]

List of cell lines

Cell linePrimary tumorOrigin of cellsEstrogen receptorsProgesterone receptorsERBB2 amplificationMutated TP53[Notes 2]Tumorigenic in miceReferenceExternal links
600MPEInvasive ductal carcinoma+[3]Cellosaurus
AMJ13Invasive ductal carcinomaPrimaryYesYesNo--[4]Cellosaurus
BT-20Invasive ductal carcinomaPrimaryNoNoNoYesYes[5]Cellosaurus
BT-474Invasive ductal carcinomaPrimaryYesYesYesYesYes[6]Cellosaurus
BT-483Invasive ductal carcinoma++[3]Cellosaurus
BT-549Invasive ductal carcinoma+[3]Cellosaurus
Evsa-TInvasive ductal carcinoma, mucin-producing, signet-ring typeMetastasis (ascites)NoYes?Yes?[7]Cellosaurus
MCF-7Invasive ductal carcinomaMetastasis (pleural effusion)YesNo[9]NoNo (wild-type)Yes (with estrogen supplementation)[10]Cellosaurus
MDA-MB-231Invasive ductal carcinomaMetastasis (pleural effusion)NoNoNoYesYes[11]Cellosaurus
SkBr3Invasive ductal carcinomaMetastasis (pleural effusion)NoNoYesYesNo[12]Cellosaurus
T-47DInvasive ductal carcinomaMetastasis (pleural effusion)YesYesNoYesYes (with estrogen supplementation)[13]Cellosaurus
ZR-75-1Invasive ductal carcinoma[14]Cellosaurus


  1. The original list was mainly based on the work of Lacroix and Leclercq (2004).[1]
  2. For more data on the nature of TP53 mutations in breast cancer cell lines, see Lacroix et al. (2006).[2]


  1. Lacroix M, Leclercq G (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Cancer Res Treat. 83 (3): 249–289. doi:10.1023/ PMID 14758095.
  2. Lacroix M, Toillon RA, Leclercq G (2006). "p53 and breast cancer, an update". Endocrine-Related Cancer. 13 (2): 293–325. doi:10.1677/erc.1.01172. PMID 16728565.
  3. Neve RM, et al. (2006). "A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes". Cancer Cell. 10 (6): 515–527. doi:10.1016/j.ccr.2006.10.008. PMC 2730521. PMID 17157791.
  4. Al-Shammari A, Alshami M, Umran M, Almukhtar A, Yaseen N, Raad K, Hussien A (2015). "Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient". Breast Cancer: Targets and Therapy. 2015 (7): 223–230. doi:10.2147/BCTT.S74509. PMC 4536763. PMID 26300657.
  5. Lasfargues EY, Ozzello L (1958). "Cultivation of human breast carcinomas". Journal of the National Cancer Institute. 21 (6): 1131–1147. PMID 13611537.
  6. Lasfargues EY, Coutinho WG, Redfield ES (1978). "Isolation of two human tumor epithelial cell lines from solid breast carcinomas". Journal of the National Cancer Institute. 61 (4): 967–978. doi:10.1093/jnci/61.4.967. PMID 212572.
  7. Borras M, Lacroix M (1997). "Estrogen receptor-negative/progesterone receptor-positive Evsa-T mammary tumor cells: a model for assessing the biological property of this peculiar phenotype of breast cancers". Cancer Letters. 120 (1): 23–30. doi:10.1016/S0304-3835(97)00285-1. PMID 9570382.
  8. Hackett AJ, Smith HS, Springer EL, Owens RB, Nelson-Rees WA, Riggs JL, Gardner MB (1977). "Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines". Journal of the National Cancer Institute. 58 (6): 1795–1806. doi:10.1093/jnci/58.6.1795. PMID 864756.
  9. Kao, Jessica; Salari, Keyan; Bocanegra, Melanie; Choi, Yoon-La; Girard, Luc; Gandhi, Jeet; Kwei, Kevin A.; Hernandez-Boussard, Tina; Wang, Pei (2009-07-03). "Molecular Profiling of Breast Cancer Cell Lines Defines Relevant Tumor Models and Provides a Resource for Cancer Gene Discovery". PLOS ONE. 4 (7): e6146. Bibcode:2009PLoSO...4.6146K. doi:10.1371/journal.pone.0006146. ISSN 1932-6203. PMC 2702084. PMID 19582160.
  10. Soule HD, Vazguez J, Long A, Albert S, Brennan M (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute. 51 (5): 1409–1416. doi:10.1093/jnci/51.5.1409. PMID 4357757.
  11. Cailleau R, Young R, Olivé M, Reeves WJ Jr (1974). "Breast tumor cell lines from pleural effusions". Journal of the National Cancer Institute. 53 (3): 661–674. doi:10.1093/jnci/53.3.661. PMID 4412247.
  12. Engel LW, Young NA (1978). "Human breast carcinoma cells in continuous culture: a review". Cancer Research. 38 (11 Pt 2): 4327–4339. PMID 212193.
  13. Keydar I, Chen L, Karby S, Weiss FR, Delarea J, Radu M, Chaitcik S, Brenner HJ (1979). "Establishment and characterization of a cell line of human breast carcinoma origin". European Journal of Cancer. 15 (5): 659–670. doi:10.1016/0014-2964(79)90139-7. PMID 228940.
  14. Dorssers, LC; van Agthoven, T (July 1996). "Genetic mechanisms of estrogen-independence in breast cancer". Pathology, research and practice. 192 (7): 743–51. doi:10.1016/S0344-0338(96)80096-3. PMID 8880875. Retrieved 2019-11-28.
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