Heller's test

Heller's test is a chemical test that shows that strong acids cause the denaturation of precipitated proteins. Concentrated nitric acid is added to a protein solution from the side of the test tube to form two layers. A white ring appears between the two layers if the test is positive.[1] Heller's test is commonly used to test for the presence of proteins in urine.[2] This test was discovered by the Austrian Chemist, Johann Florian Heller (1813-1871).


  1. Dandekar (1 January 2004). Practicals And Viva In Medical Biochemistry. Elsevier India. p. 26. ISBN 978-81-8147-025-6.
    - Nigam (1 April 2007). Lab Manual in Biochemistry: Immunology and Biotechnology. Tata McGraw-Hill Education. p. 152. ISBN 978-0-07-061767-4.
    - Chawla (1 January 2003). Practical Clinical Biochemistry: Methods and Interpretations. Jaypee Brothers Publishers. p. 59. ISBN 978-81-8061-108-7.
    - A.C. Croftan. Clinical Urinology. Рипол Классик. p. 115. ISBN 978-1-275-01265-3.
  2. Elizabeth A. Martin (ed) (25 February 2010). Concise Colour Medical Dictionary. Oxford University Press. p. 335. ISBN 0-19-955715-2.CS1 maint: extra text: authors list (link)
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