Hay's test

Hay's test, also known as Hay's sulphur flower test, is a chemical test used for detecting the presence of bile salts in urine.[1]


Sulphur powder is sprinkled into a test tube with three millilitres of urine and if the test is positive, the sulphur powder sinks to the bottom of the test tube. Sulphur powder sinks because bile salts decrease the surface tension of urine.[2][3][4][5]


  1. D M Vasudevan (2013). Textbook of Biochemistry for Medical Students. JP Medical Ltd. p. 350. ISBN 978-93-5090-530-2.
  2. Ashok Kumar J (1 January 2007). Textbook of Biochemistry for Nurses. I. K. International Pvt Ltd. p. 208. ISBN 978-81-89866-45-7.
  3. Harsh Mohan (30 November 2012). Pathology Practical Book. JP Medical Ltd. p. 169. ISBN 978-93-5090-266-0.
  4. Dandekar (1 January 2004). Practicals And Viva In Medical Biochemistry. Elsevier India. p. 47. ISBN 978-81-8147-025-6.
  5. Chary (1 January 2004). Practical Biochemistry for Medical and Dental Students. Jaypee Brothers Publishers. p. 20. ISBN 978-81-8061-233-6.
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