Bicinchoninic acid

Bicinchoninic acid /bsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid
Names
IUPAC name
2-(4-Carboxyquinolin-2-yl)quinoline-4-carboxylic acid
Other names
Bicinchoninic acid
4,4'-Dicarboxy-2,2'-biquinoline
Identifiers
3D model (JSmol)
ChEMBL
ChemSpider
ECHA InfoCard 100.013.628
Properties
C20H12N2O4
Molar mass 344.326 g·mol−1
Appearance Cream colored powder
Odor Characteristic odor
Melting point 365 to 367 °C (689 to 693 °F; 638 to 640 K)
Partially soluble in cold water, hot water
Hazards
Main hazards Will irritate eyes and mucous membranes.
Safety data sheet External MSDS
NFPA 704 (fire diamond)
1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[1]

References

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