Acree–Rosenheim reaction

The Acree-Rosenheim reaction is a chemical test used for detecting the presence of tryptophan in proteins. A protein mixture is mixed with formaldehyde. Concentrated sulfuric acid is added to form two layers. A purple ring appears between the two layers if the test is positive for tryptophan.[1][2][3]

The test was named after two greats in biochemistry, namely, Solomon Farley Acree (18751957), a distinguished American Biochemist at Johns Hopkins University and Sigmund Otto Rosenheim (18711955), an Anglo-German Medical Chemist at the University of Manchester.


  1. Debajyoti Das (1980). Biochemistry. Academic Publishers. p. 56. ISBN 978-93-80599-17-5.
  2. Louis Rosenfeld (2 December 2012). Origins of Clinical Chemistry: The Evolution of Protein Analysis. Elsevier. p. 76. ISBN 978-0-323-15292-1.
  3. B. Jain Publishers Staff (1 January 1999). Pocket Medical Dictionary. B. Jain Publishers. p. 16. ISBN 978-81-7021-193-8.
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