Acholeplasma are wall-less bacteria in the Mollicutes class. They include saprotrophic or pathogenic species. There are 15 recognised species. The G+C content is low, ranging from 26 - 36% (mol%). The genomes of Acholeplasma species range in size from 1.5 to 1.65 Mbp. Cholesterol is not required for growth. The species are found on animals, and some plants and insects. The optimum growth temperature is 30 to 37 degrees Celsius.[1]

Scientific classification

Edward & Freundt 1970

Acholeplasma axanthum
Acholeplasma brassicae
Acholeplasma cavigenitalium
Acholeplasma entomophilum
Acholeplasma equifetale
Acholeplasma florum
Acholeplasma granularum
Acholeplasma hippikon
Acholeplasma laidlawii
Acholeplasma modicum
Acholeplasma morum
Acholeplasma multilocale
Acholeplasma oculi
Acholeplasma palmae
Acholeplasma parvum
Acholeplasma pleciae
Acholeplasma seiffertii
Acholeplasma vituli

Acholeplasma laidlawii is a common contaminant of cell culture media products, and has also been used in extensive studies of lipid polymorphism because this organism alters its ratio of MGlcDG (monoglucosyl diacylglycerol) to DGlcDG (diglucosyl diacylglycerol) in response to growth conditions.[2]


  1. Windsor, Helena M.; Windsor, G. David; Noordergraaf, J.H. (2010). "The growth and long term survival of Acholeplasma laidlawii in media products used in biopharmaceutical manufacturing". Biologicals. 38 (2): 204. doi:10.1016/j.biologicals.2009.11.009. PMID 20153666.
  2. Vance. Biochemistry of Lipids, Lipoproteins and Membranes. p. 18. ISBN 978-0444532190.

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