ATP synthase-coupling factor 6, mitochondrial is an enzyme that in humans is encoded by the ATP5PF gene.[5][6][7]

AliasesATP5PF, ATP5, ATP5A, ATPM, CF6, F6, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6, ATP synthase peripheral stalk subunit F6, ATP5J
External IDsOMIM: 603152 MGI: 107777 HomoloGene: 1272 GeneCards: ATP5PF
Gene location (Human)
Chr.Chromosome 21 (human)[1]
Band21q21.3Start25,716,503 bp[1]
End25,735,673 bp[1]
RNA expression pattern
More reference expression data









RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)Chr 21: 25.72 – 25.74 MbChr 16: 84.83 – 84.84 Mb
PubMed search[3][4]
View/Edit HumanView/Edit Mouse
Mitochondrial ATP synthase coupling factor 6 (F6)
solution structure of subunit f6 from the peripheral stalk region of atp synthase from bovine heart mitochondria


Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the F0 complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.[7]

The F6 subunit is part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In mitochondria, the peripheral stalk is composed of one copy each of subunits OSCP (oligomycin sensitivity conferral protein), F6, B and D.[8] There is no homologue of subunit F6 in bacterial or chloroplast F-ATPase, whose peripheral stalks are composed of one copy of the delta subunit (homologous to OSCP), and two copies of subunit B in bacteria, or one copy each of subunits B and B' in chloroplasts and photosynthetic bacteria.


  1. GRCh38: Ensembl release 89: ENSG00000154723 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000022890 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Higuti T, Tsurumi C, Kawamura Y, Tsujita H, Osaka F, Yoshihara Y, Tani I, Tanaka K, Ichihara A (Jul 1991). "Molecular cloning of cDNA for the import precursor of human coupling factor 6 of H(+)-ATP synthase in mitochondria". Biochemical and Biophysical Research Communications. 178 (2): 793–9. doi:10.1016/0006-291X(91)90178-A. PMID 1830479.
  6. Javed AA, Ogata K, Sanadi DR (Jan 1991). "Human mitochondrial ATP synthase: cloning cDNA for the nuclear-encoded precursor of coupling factor 6". Gene. 97 (2): 307–10. doi:10.1016/0378-1119(91)90068-M. PMID 1825642.
  7. "Entrez Gene: ATP5PF ATP synthase peripheral stalk subunit F6".
  8. Carbajo RJ, Kellas FA, Runswick MJ, Montgomery MG, Walker JE, Neuhaus D (Aug 2005). "Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit". Journal of Molecular Biology. 351 (4): 824–38. doi:10.1016/j.jmb.2005.06.012. PMID 16045926.

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR008387

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